ASYMMETRIC NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION (ASYMMETRIC-NASBA): A DIFFERENT METHOD OF CONVENTIONAL NASBA

摘要：

Nucleic acid sequence-based amplification (NASBA) is a homogeneous, isothermal, in vitro amplification resulting in the amplification of target sequences more than 109-fold within 120 min1. The main amplicons of NASBA are RNA, and currently all the detection method are designed for RNA. In this paper, asymmetric NASBA with unequal concentrations of T7 promotor primer and reverse transcription primer was proposed. By optimizing the ratio of two primers and the concentration of dNPTs and NTPs, the amount of single-stranded cDNA in the amplicons from asymmetric NASBA was found increased 12 times more through the real-time detection of molecular beacon specific to cDNA of interest

关键词： NASBY asymmetric

作者: Jia Huning Chen Zhiyao Wu Haiping Ye Hui Zhou Guohua

作者单位: Department of Analytical Chemistry , China Pharmaceutical University, Nanjing 210009,China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China Medical School, Nanji Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China School of life science and technology, China Pharmaceutical University, Nanjing 210009, China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 253-255

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）