CLONING AND CHARACTERIZATION OF A NOVEL METALLOTHIONEIN cDNA FROM KANDELIA CANDEL
A new metallothionein (MT) gene named KMT was cloned from Kandelia candel, a mangrove plant with constitutional tolerance to heavy metals, by rapid amplification of cDNA ends (RACE). The full length of KMT cDNA was 744 bp including 120 bp 5 noncoding domain, 240 bp open reading frame and 384 bp 3 termination. The characters of KMT protein showed it was 79aa in length, the molecular weight was 7. 75 kDa, the pi was 4. 81, and 14 cysteine motifs were detected to distribute at both N-and C-terminals which were thought to be the putative metal irons binding domain. The phylogenetic investigation indicated that the KMT protein sequence shared more than 60％ homology with the type 2 MT-like protein genes of other higher plants. At amino acid level, the KMT was shown homology with the MT of Quercus suber (83％), of Ricinns communis (81％) and of Arabidopsis thaliana (64％).
Metallothionein gene kandelia candel the full length of cDNA Homology
Zhang FengQin Wang YouShao Li Xiaolong He NongYue
Key Laboratory of Green Packaging and Biological Nanotechnology, Hunan Province,Hunan University of South China Sea Institute of Oceanology, Chinese Academy of Sciences, Marine Environment & Ecology D
国际会议
重庆
英文
260-262
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）