CLONING OF FULL-LENGTH HUMAN ESTROGEN RECEPTOR AND CONSTRUCTION OF THE EXPRESSION VECTOR IN YEAST

摘要：

To establish the recombinant yeast estrogen system, cloning and expression of full-length human estrogen receptor(hER) cDNA in yeast The hER cDNA was amplified by RT-PCR method from human breast carcinoma cells,MCF-7, according to the sequence of human full length of estrogen recepor cDNA. The gene was cloned into the yeast expression vector via pPIC9/hER vector, which was transformed into Pichia pastoris. The result showed that recombinant expression vector of full-length human enstrogen recepor cDNA, pPIC9/hER, was constructed successfully, which was the very important groundwork for further establishment of the recombinant yeast estrogen system.

关键词： Environmental estrogen hormone Estrogen receptor Yeast

作者: Zheng Chenna Yang Huiyong Li Qingsong Yang Li Zeng Shulan Diao Yong

作者单位: Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, China Water Resources and Environmental Institute,Xiamen University of Technology,Xiamen 361000,China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 263-265

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）