CONSTRUCTION OF PLASMID CONTAINING HUMAN ARGONAUTE2 GENE

摘要：

Objective To construct recombinant plasmid for further studying the biological function of the Human Argonautez Gene. Methods The total RNA was extracted from HeLa cells. The cDNA encoding human full-length Argonautez (AGO2) gene was obtained by RT-PCR. The AGO2 gene was subcloned to Fluorescent Protein Expression Vector pKaded-MNl to construct recombinant expression vector named pKaded-MNl/AGO2. The proper cloning was identified by electrophoresis and confirmed by sequencing. Results Restriction endonuclease digestion analysis, sequencing showed that the target gene Argonauts was cloned into pKaded-MN1. Conclusion The recombinant plasmid containing Human Argonautez gene has been achieved successfully.

关键词： Argonautez recombinant plasmid construction

作者: Chen Shanshan Zeshan Basit Tian Tian Wang Yuanyuan Liu Yongping Xiao Zhongdang

作者单位: State Key laboratory of Biomedical Electronics, School of Biological Science and Medical Engineering,Southeast University, Nanjing 210096, China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 275-277

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）