DEVELOPMENT OF MULTIPLEX LOOP MEDIATED ISOTHERMAL AMPLIFICATION METHOD FOR THE SIMULTANEOUS DETECTION OF HBV, HCV, HIV AND TP
Loop-mediated isothermal amplification (LAMP) technique has been used as a novel nucleic acid detection method, whereby the target DNA can be amplified with high specificity and sensitivity under an isothermal condition using a set of four or six specific primers. In this study, we designed four sets of the LAMP primers for conservative sequences of HBV, HCV, HIV and TP, which construct a multiplex LAMP(mLAMP) method by combining these four sets totaling 32 primers. The mLAMP method was distinguishable among HBV, HCV, HIV and TP, simultaneously, due to the subsequent restriction enzyme analysis. The sensitivities of the mLAMP method were 10 to 100 times higher on the detection limits for HBV, HCV, HIV and TP respectively, than those of the classical PCR methods. Of 164 blood samples collected from China, 104 were positively detected by the mLAMP for HBV, 30 for HCV, 20 for HIV and 10 for TP. These findings indicate that this mLAMP method is a new convenient tool for simultaneous detection of the pathogens in blood.
multiplex LAMP restriction enzyme analysis blood pathogens
Liang Chao Cheng Sijia Chu Yanan Zhou Guohua
Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China College of Life Scien Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China
国际会议
重庆
英文
281-284
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）