DOWN-REGULATION OF MICRORNA139-5P IN ESOPHAGEAL SQUANMOUS CELL CARCINOMA
Objectives To explore the expression profile and the potential role of miR-139-5p in esophageal squamous cell carcinoma (ESCC). Methods: Quantitative realtime PCR was used to evaluate the expression level of miR-139-5p in 137 pairs of ESCC tissues and corresponding normal tissues. Genome-wide microarray was carried out on miR-139-5p mimics transferred EC109 cells and control Genes differently expressed by miR-139-5p over-expression were distributed to GO and pathway analysis. Results: miR-139-5p expression level in ESCC tissues was 9. 43-fold lower than adjacent tissues (P<0. 000 1). Totally 74 upregulated (Ratio>2) and 136 down-regulated mRNAs (Ratio<0. 5) were identified as a consequence of miR139-5p over-expression, which were mostly linked to protein phosphorylation, oxidation reduction and biding process, and were involved in several pathways critical related to carcinogenesis, including apoptosis, MAPK and TGF-β signaling pathways. Conclusion: miR-139-5p was significantly down-regulated in ESCC tissues. Over-expression of miR-139-5p in ESCC cell may trigger large amounts of downstream events and reduce the risk of esophageal cancer as tumor-suppressor gene by being involved in apoptosis, MAPK and TGF-β signaling pathways.
Esophageal squamous cell carcinoma microRNA miR-139-5p microarray
Sheng Jingyi Liu Ran Yang Miao Liao Juan Yin Lihong
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health,Southeast University, Nanjing, China
国际会议
重庆
英文
288-291
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）