DYNAMICS STUDIES OF EXOGENOUS MIRNA TRANSFECTION BY LIVE-CELL MICROSCOPY AND SINGLE PARTICLE TRACKING

摘要：

In recent years, microRNAs (miRNAs) have emerged as important gene-regulators and showed great potential in gene therapy. However, the knowledge of their function in vivo is still lacking. In this work, the exogenous miR-21 transfected into HeLa cells was observed for a long time by live-cell microscopy. Their dynamic properties were analyzed by single particle tracking (SPT) and mean square displacement (MSD) calculation. The results showed that the exogenous miRNAs bound to cells quickly and were confined in a small area. Then long time observation and the analysis of motion mode showed they entered cells and changed their motion mode in different intracellular microenvironment.

关键词： MicroRNA Single particle tracking Live-cell microscopy

作者: Wang Yuanyuan Tian Tian Xiao Zhongdang

作者单位: State Key laboratory of Biomedical Electronics, School of Biological Science and Medical Engineering,Southeast University, Nanjing 210096, China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 292-294

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）