EXPRESSION OF MIRNAS RELATIVED WITH ANGIOGENESIS AND VEGF SIGNALING IN RESPONSE TO HYPOXIA IN HUVEC
miRNAs (MicroRNAs) are a class of endogenous, conserved, small non-coding RNAs which play important regulatory roles in many physiological processes. Here we sought to investigate potential involvement of miRNAs relatived with angiogenesis in HUVEC under hypoxia. Quantitative Real-time RT-PCR were performed to identical miRNA changes in hypoxic cells. miRNA210 and miRNA-126 expression increased to (4. 73±0. 55) and (5. 22±0. 71)folds respectively, while miRNA-320 expression decreased to (0. 29 ± 0. 10) folds significantly after hypoxia (P<0. 05 ). VEGF and VEGFR2 mRNA expression and proteinwere increased after hypoxia compared with control group detected by Quantitative Real-time RT-PCR, ELISA andFlow Cytometry respectively(P< 0. 05). In conclusion, our results imply that miRNAsmay be involved in targeting VEGF pathway signaling to regulate angiogenesis after hypoxia in vitro. These results provide novel insights into the angiogenesis mechanism of renal I/R injury.
MicroRNA Angiogenesis Hypoxia HUVEC
Liu Fen Lou Yuanlei Xie An Yang Yang Li Yong Wang Yang
Institute of Urology the First Affiliated Hospital of Nanchang University, Nanchang 330006, China Department of oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China Institute of Urology the First Affiliated Hospital of Nanchang University, Nanchang 330006, China In
国际会议
重庆
英文
298-300
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）