GENETICALLY MODIFIED ORGANISMS ANALYSIS BY PYROSEQUENCING ON A PORTABLE PHOTODIODE-BASED BIOLUMINESCENCE ANALYZER

摘要：

In this paper, we developed an inexpensive bioluminescence analyzer by using a photodiode (PD) array; pyrosequencing with this instrument was successfully performed. The DNA sequence of genetically modified organisms (GMO): Bt-11, Bt-176 Maize and Roundup ready soya were successfully detected The corresponding limit of detection (LOD) was 0. 01 ％ with 35 PCR cycles. The GMO test result and the small volume of the detector have immense potential for use as a farm/field test

关键词： Genetically Modified Organisms (GMO) Bt-11 and Bt-176 Maize Roundup ready soya Pyrosequencing Portable Bioluminescence Analyzer

作者: Song Qinxin Zhou Guohua

作者单位: China Pharmaceutical University, Nanjing 210009, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China Medical School, Nanji

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 307-309

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）