HYDROGEL MICROARRAY FOR LOW-COST SNP SCREENING
This paper propoed a hydrogel chip platform to established a low-cost method for screening of SNP. The proposed method based on copolymerization of acrylamideamidemodified PCR products with acrylamideamide monomers and acrylamidemodified slides to prepare gelbased microarray. Then the array was hybridized with two types of hybridization probes for SNP respectively. Electrophoresis was used to remove the nonspecifically bound targets and mismatches. After that, the array was extended with dNTPs containing Cy5-dCTP. Finally, genotyping was based on fluorescence scanning. As the using of general probe during the hybridization, the specific labeled probesis non-essential for each SNPwhich can reduce the cost of screening. 16 samples of OLR1C14417G were detected, the results are consistent with Sequencing. The established hydrogel chip platform is a low-cost method for detection of SNP.
Hydrogel chip single nucleotide polymorphisms specific primer extension
Wang Hui Chen Zhiyao Li Youzhi Huang Huan Zhou Guohua
Huadong Research Institute for Medicine and Biomechanics, Nanjing, China Nanjing Medical University, Huadong Research Institute for Medicine and Biomechanics, Nanjing, China School of Medicine, Nanjing Huadong Research Institute for Medicine and Biomechanics, Nanjing, China Huadong Research Institute for Medicine and Biomechanics, Nanjing, China Nanjing Medical University,
国际会议
重庆
英文
313-315
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）