MICRORNAS AND THE UPREGULATION OF CYTOGLOBIN IN ACTIVATED HEPATIC STELLATE CELLS
The expression of cytoglobin (Cygb) is upregulated during hepatic stellate cells (HSCs) activation which was speculated to be closely related to the progress of liver fibrosis, but its mechanisms still remain to be revealed. microRNA (miRNA)-mediated RNA interference might be a novel mechanism underlying the regulation of Cygb expression in activated HSCs. Bioinformatics approaches were used to anticipate the possible miRNAs targeting Cygb prior to the miRNA microarray analysis. 5 miRNAs were calculated based on bioinformatics approaches to target rat Cygb mRNA by three databases simultaneously. Among them, the levels of miR-17 and miR-20b-5p were significantly downregulated in activated HSCs rather than quiescent ones, while miR-106b, miR-93 and miR-96 showed no significant change by miRNA microarray analysis. On the whole downregulation of these five miRNAs indicated that the expression of Cygb might be regulated, at least in part, by miRNAs at the translational level
cytoglobin hepatic stellate cells activation microRNA
Lv Yinghui Wang Qizhao Diao Yong Xu Ruian
Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, China
国际会议
重庆
英文
328-330
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）