MIR-210 UP-REGULATES VEGF SIGNALING FOR ANGIOGENESIS IN VITRO
MicroRNAs(miRNA) are short non-coding RNA molecules that negatively regulate gene expression by posttranslational suppression or target mRNA degradation in a sequence-specific manner. In the present study, we investigated miRNA role in regulating angiogenesis in vitro and explored the underlying mechanisms involved in these events, we found that overexpression of miR-210 in human umbilical vein endothelial cells (HUVE-12) by molecular cloning technology stimulated the formation of capillary-like structures on Matrigel . To elucidate the further mechanisms of miR-210 regultion of angiogenesis, we analyzed the expression of VEGF /VEGFR2 at both mRNA and protein levels in HUVEC-12 cell line after miR210 overexpression. The rsults showed that VEGF/VEGFR2 expression in cells overexpression miR210 were markedly up-regulated compared with that in control. It suggested that VEGF signaling pathway may be involved in the regulation mechanism of miR-210mediated angiogenesis.
miR-210 VEGF angiogenesis
Lou Yuanlei Deng Zhifeng Guo Fei Liu Fen Xie An Cui Suping Wang Yang
Institute of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006,
国际会议
重庆
英文
334-336
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）