MRNA ANALYSIS OF MULTICELLULAR SPHEROID WITH A SCANNING PROBE MICROSCOPY SYSTEM
Here, we introduce several applications for singlecell gene-expression analysis utilizing electrical cell lysis technique with a Pt-ring electrode probe. Glass capillary probes featuring a Pt-ring electrode were utilized to collect single cell with two or three dimensional culture systems. An electric pulse with 100 V height and 10 μs width was applied to lyse the cellular membrane of the single cell in 0. 2 M sucrose solution. Multicellular spheroid of human breast cancer cell line MCF-7 was formed according to hanging drop method or on-top culture on a matrigel sheet. It was shown that evaluation of mRNA expression level is possible at single-cell level collected at the surface of the multicellular spheroid.
Single cell analysis mRNA scanning probe microscopy multifunctional electrode probe spheroid
Hitoshi Shiku Genki Saito Yuanshu Zhou Yoshiko Horiguchi Ryosuke Takano Kosuke Ino Tomokazu Matsue
Graduate School of Environmental Studies, Tohoku University, Aramaki-Aoba 6-6-11-604,Sendai 980-8579 Graduate School of Environmental Studies, Tohoku University, Aramaki-Aoba 6-6-11-604,Sendai 980-8579
国际会议
重庆
英文
343-344
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）