PREPAREATION OF MONOVALENT CIRCULAR SSDNA APTAMER BY ENZYME LIGATION
Nucleic-acid aptamer is a promising therapeutic agent The instability of aptamer in vivo limits its application in clinical therapy, whereas it could be improved by means of modification. Circular aptamer is a promising modification method. Here we described the construction of monovalent circular ssDNA aptamer with ligation primer by T4 DNA Ligase-mediated ligation reaction. Rolling circle amplication (RCA) was used to detect circular aptamer. The obtained circular aptamer was mainly monovalent, possessed nuclease-resistant properties. T4 DNA Ligase-mediated ligation method and RCA method were simple, cheap, fast methods to prepare monovalent circular aptamer and monitor circularization reactioa
Aptamer Circularization Exonuclease Degradation
Yang Li Diao Yong Yang Huiyong
Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, China Molecular Medicine Engineering Research Center of the Ministry of Education, Huaqiao University,Quanzhou 362021, China
国际会议
重庆
英文
366-368
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）