RAPID SCREENING AND GENOTYPING OF AVIAN INFLUENZA VIRUS BY VISUAL ISOTHERMAL AMPLIFICATION
A rapid assay for nuclear-acid detection was developed to screen the avian influenza virus and three subtypes including H5N1, H7N7, and H9N2 Methods. A conserved region of the M gene in all avian influenza viruses was amplified to screen the avian influenza virus. The subtype of avian influenza virus was further identified by amplify a subtypespecific sequence of HA gene. The assay was performed by a highly sensitive loop-mediated isothermal amplification (LAMP) incubated at 60℃ for lh with three pairs of LAMP primers and Bst DNA polymerase. The visual detection was readily realized through the incorporation of SYBR Green I fluorescence to the LAMP reaction system. The sensitivity for the detection of three subtypes is 10 DNA copies per tube, and there is no non-specific amplification among the detection of the three virus subtypes.
Loop-mediated isothermal amplification(LAMP) H5N1 H7N7 H9N2
Chu Yanan Cheng Sijia Liang Chao Zhou Guohua
Huadong Research Institute for Medicine and Biotechnics, Nanjing, China China Pharmaceutical Univers Huadong Research Institute for Medicine and Biotechnics, Nanjing, China Huadong Research Institute for Medicine and Biotechnics, Nanjing, China Medical School, Nanjing Univ
国际会议
重庆
英文
374-375
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）