SIGNAL AMPLIFICATION BASED ON AFU FLAP ENDONUCLEASE COUPLED WITH NICKING ENDONUCLEASE FOR ULTRASENSITIVE DNA DETECTION
Detection of specific sequences in target DNA without contamination of amplicons is very critical to clinical diagnosis. In this report, a cascade enzymatic signal amplification method (termed as CESA) was proposed for ultrasensitive DNA detection by coupling Afu flap endonuclease with nicking endonuclease. The sensitivity of the method is as high as 1 fM DNA. Because CESA is based on a real signal amplification, no risk of cross-contamination of amplicons is achieved; thus the application of CESA to the in-situ rapid diagnosis of infectious disease out of a regular lab is much suitable. In contrast to conventional NESA-based amplification, CESA can detect any target of interest Due to a constant temperature reaction, an inexpensive instrumentation of CESA can be readily achieved by employing a simple heater with a temperature controller. All advantages above should enable CESA having a great potencial in clinical diagnosis.
signal amplification Afu flap endonuclease nicking endonuclease
Zou Bingjie Ma Yinjiao Wu Haiping Zhou Guohua
Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China Department of pharmac Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China
国际会议
重庆
英文
381-383
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）