SIRNAS-MEDIATED KNOCKDOWN OF F GENE INHIBITS THE TARGET CELL GROWTH AND ITS ANCHORAGE-INDEPENDENT GROWTH
F protein was synthesized from the initiation codon of core protein by a + 1 ribosomal frame shift, which might participate in HCV infection and hepatocarcinoma. In this study, short interfering RNAs (siRNAs) were used to silence F gene expression in artificial F-protein stable expressed cells—NIH3T3-F cells. NIH3T3-F cells were transfected with siRNAs-F (siRNAs targeting F gene) followed by analysis target cell proliferation. Furthermore, anchorage-independent growth in soft agar by siRNAs-F treatment was investigated. It was found that the siRNAs-F-mediated inhibition of F gene reduced the NIH3T3-F cell growth and led cells to lose the proliferative focus formation in soft agar. Therefore, since F protein might have diverse biological properties in natural HCV infection or even carcinogenesis, the specific knockdown of F gene might be useful in analyzing its function. The using of siRNAs-F presented appropriate tools for this aim since they reduced the expression of target F gene significantly.
F protein short interfering RNAs (siRNAs) cell proliferation anchorage-independent growth
Kong Jing Deng Xiaozhao Chu Chunli Zhang Jinhai Yue Ming Lu Weidong Zhang Yun
Huaiyin Institute of Technology, Huaian 223003, China Huadong Research Institute for Medicine and Bi Huadong Research Institute for Medicine and Biotechniques, Nanjing 210002, China Kunming Medical College, Kunming 650031, China
国际会议
重庆
英文
390-392
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）