THE REGULATION OF ANGIOGENESIS BY MICRORNA-210-MEDIATED HIF-1α/VEGF SIGNALING PATHWAYS AFTER RENAL ISCHEMIA/REPERFUSION INJURY
Neovascularization is a major physiological response to ischemia, but the regulatory mechanism of renal angiogenesis after ischemic injury remains to be undetermined. MicroRNAs are a group of small noncoding RNAs with modulator activity of gene expression. This study aimed to observe the expression of microRNA-210-mediated HIF-la/VEGF signaling pathways and explore the regulatory mechanism of the angiogenesis after ischemiareperfusion (I/R) injury. Quantitative Real-Time RTPCR analysis showed that miR-210, HIF-1αmRNA and VEGFmRNA in IR 4h and Id group were increased significantly compared to sham-operated control group. Western blot analysis also showed that HIF-lot protein in IR 4h and Id group were increased apparently compared to sham-operated control group. (w=5 each, * P<0. 05 vs. control) Thus, we conclude that The renal ischemia/reperfusion injury might put an influence on the expression of miR-210, HIF-la,VEGF. The regulation of angiogenesis after renal ischemia/reperfusion injury might be associated with the microRNA-210-mediated HIF-la/VEGF signaling pathways.
Renal Ischemia-reperfusion injury HIF-1α/VEGF signaling pathways MicroRNA-210 Angiogenesis
Li He Wang Yang Liu Fen Deng Jun Lou Yuanlei Guo Fei Xie An Cui Suping
Institute of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China Institute of Orthopaedic Surgery,Shanghai Sixth Peoples Hospital,Shanghai Jiao-tong University,Shan
国际会议
重庆
英文
409-411
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）