TN-SEQ: A ROBUST TECHNOLOGY FOR CHARACTERIZATION OF GENE FUNCTION IN POST-GENOME STAGE
Characterization of gene function and construction of complex network of interacting genes are two major challenges in understanding biological systems. Transposon-based saturated mutation is one of the most powerful technologies for defining the genetic basis of complex traits. However, genome-wide identification of insertion locus is laborious, even impractical. A novel technology, termed Tn-seq, which combines high-density transposon mutagenesis and high-throughput insertion tracking by deep sequencing, has provided dramatic improvement in characterization of gene functioa Tn-seq is composed of several steps, including generation of mutants by transposon, isolation of genomic DNA, selective enrichment of fragments that contain transposongenome junctions, and deep sequencing. The application of Tn-seq has resulted in identification of a number of new genes involved in a variety of traits in several species, such as antibiotic resistance in Pseudomonas aeruginosa, mutant interactions in Streptococcus pneumoniae.
gene transposon deep sequencing Tn-seq
Liao Guojian Wang Lei Hu Changhu
Colllege of Pharmaceutic Science, Southwest University, Chongqing 400716, China
国际会议
重庆
英文
412-414
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）