CYTOTOXICAL EFFECT OF N-METHYL-N-NITRO-N-NITOOSOGUANTOINE ON MGMT DEFICIENT ESOPHAGEAL EPITHELIAL CELLS WAS RELATED TO THE INDUCED EXPRESSION OF HMSH2
Objective-To detect the responsive expression of hMSH2 and hMLHl in MGMT deficient and proficient cells exposed to N-methyl-Nt-nitro-N-nitrosoguanidine (MNNG) and explore the relation between the responsive reaction and MNNG cytotoxicity. Methods: RNA interference technology was introduced to silence MGMT expression in a normal esophageal epithelial cell line (H5E46) and quantitative real-time reverse transcription PCR and western-blot were employed to evaluate the silence effect MTT was used to measure cytotoxical effect of MNNG. Results: The IC50 of MGMT deficient and proficient cells was 20 μM and 50 μM, respectively; the transcriptional and protein expression level of hMSH2 is statistically higher in MGMT deficient cells and a doseresponse relationship was detected from 10 μM to 40 μM; however, no significant variation of hMLHl expression was detected in the two cells; Conclusion: the loss expression of MGMT may result in the compensatory expression of hMSH2 in H5E46 cells when exposed to MNNG, which might result in the higher sensitivity to the cytotoxicity of MNNG.
MGMT hMSH2 hMLHl RNAi MNNG
Su Yaoyao Liu Ran Sheng Jingyi Liu Hui Yang Miao Liao Juan Pu Yuepu Yin Lihong
Key Laboratory of Environmental Medicine Engineering, Ministry of Education,School of Public Health, Southeast University, Nanjing, China
国际会议
重庆
英文
434-436
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）