OPTIMIZATION OF CULTURE MEDIUM FOR THREONINE PRODUCTION OF ESCHERICHIA COLI
Threonine is an essential amino acid often used as nutritive additive in wide field. Microorganism fermentation is the most popular method currently. In this study, we investigated the culture medium using a threonine-producing E. coli strain M122. To achieve the better cell density and threonine production, the carbon and nitrogen sources were optimized. Four carbon sources including glucose, sucrose, lactose or maltose, and four nitrogen sources (ammonium sulfate, urea, peptone or yeast extract) were analyzed in this study. Data showed that the suitable carbon source for threonine production by E. coli M122 was sugar whose yield of threonine was 28.1 g/L and cell density (OD600nm) was 1.1. When ammonium sulfate or yeast extract were acted as nitrogen sources, production reached 27. 8 g/L and 28. 2 g/L, respectively. Then two of them could be used as optimal nitrogen sources. Therefore, selecting the suitable medium components can achieve the costeffective production of threonine.
threonine production carbon source nitrogen source
Gu Xu Kong Jing Jia Jianbo Zhao Yuping Li Xiangqian
Huaiyin Institute of Technology, Huaian 223003, China
国际会议
重庆
英文
454-456
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）