Physiochemical and structural characterization of biosurfactant from fluorescent Pseudomonas with biocontrol activity against Macrophomina phaseolina
Plant growth promotory fluorescent Pseudomonas EKi from the rhizosphere of tomato was screened for the production of biosurfactant. The biosurfactant production was monitored as a function of emulsification activity and cell hydrophobicity. Extracted biosurfactant was purified by thin-layer chromatography. Cell-free culture supernatant and purified biosurfactant were checked for biocontrol activity against Macrophomina phaseolina. Purified biosurfactant was characterized biochemically and by fourier transform infrared spectroscopy (FTIR) analysis. EKi showed 66.73％ of cell adherence with crude oil and emulsification activity of 67.65％ after 4 hours of cultivation, thereby confirming the biosurfactant-producing ability.
Biocontrol biosurfactant Macrophomina phaseolina Pseudomonas rhamnolipid
Ekta Khare Naveen K. Arora
Department of Microbiology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur-208024, UP, India
国际会议
The 2nd Asian PGPR Conference(第二届亚洲植物促生根际细菌会议)
北京
英文
101-106
2011-08-21（万方平台首次上网日期，不代表论文的发表时间）