Rapid assessment of the antagonistic potential of Bacillus strains against the infection with Phytophthora capsici

摘要：

Evaluation of antagonistic Bacillus strains against phytopathogens is a time-consuming and laborious work. Here we report a rapid method to assess the antagonistic potential of Bacillus strains by analysis of their biofilm formation and lipopeptide encoding genes. Of 93 Bacillus strains tested, 48 strains had ability to form biofilm on polypropylene tube walls, and were subjected to PCR amplification as the DNA sources by use of the primers specific for lipopeptide encoding genes (srfAA, srfAB, ituC and fenD). The antagonistic ability of the strains forming biofilm and/or harboring lipopeptide coding genes against the infection with Phytophthora capsici was evaluated by radicle assays on agar plates.

关键词： biofilm lipopeptide encoding genes phytophthora disease Bacillus sp

作者: Ya-Li SUO Rong-Jun GUO Shi-Dong LI Baocheng Zhu

作者单位: Key Laboratory for Biological Control of the Ministry of Agriculture, Institute of Plant Protection, Key Laboratory for Biological Control of the Ministry of Agriculture, Institute of Plant Protection, College of Life Science, Hebei Agricultural University, Baoding 071001, China

会议类型: 国际会议

会议名称: The 2nd Asian PGPR Conference(第二届亚洲植物促生根际细菌会议)

会议地点: 北京

会议语种:英文

页码: 176-181

在线出版日期: 2011-08-21（万方平台首次上网日期，不代表论文的发表时间）