Diversity analysis of culturable bacteria in the root of tree peony (Paeonia ostii)
The distribution of culturable bacteria in the bulk soil, rhizosphere, and rhizoplane of tree peony plants was investigated in this study. A total of 507 bacterial strains with different colony characteristics were isolated. There were 46 operational taxonomic units (OTUs) which were identified by amplified ribosomal DNA restriction analysis (ARDRA). Representatives of each group were selected for partial 16S rRNA gene sequencing and phylogenetic analysis. Results showed that the major bacteria group in the bulk soil, rhizosphere and rhizoplane were Firmicutes (63.2％), Actinobacteria (36.3％) and Betaproteobacteria (53.0％), respectively. The most common genus in the bulk soil were Bacillus (49.6％), in the rhizosphere were Microbacterium (21.1％) and in the rhizoplane were Variovorax (53.0％). The rhizospheric bacterial community was more diverse than the rhizoplane and bulk soil bacterial communities. Our results show that obvious differences exist among the culturable bacterial communities in the bulk soil, rhizosphere and rhizoplane. It is obviously that a selective pressure of tree peony plants on their associated bacterial populations occurred.
Culturable bacteria diversity ARDRA tree peony (Paeonia ostii)
Yao Song Jigang Han Yonghong Hu
Shanghai Engineering Research Center of Sustainable Plant Innovation, Shanghai Botanical Garden, Shanghai, China
国际会议
The 2nd Asian PGPR Conference(第二届亚洲植物促生根际细菌会议)
北京
英文
238-244
2011-08-21（万方平台首次上网日期，不代表论文的发表时间）