A Comparative Study of Modified Materials of Acetylcholinesterase Biosensor
In this study, multi-walled carbon nanotubes (MWCNTs), gold nanoparticles (GNPs) and Prussian Blue (PB) were used for modifying glassy carbon working electrode (GCE) to construct acetylcholinesterase (AChE) bio-sensor respectively. Chitosan membrane was used for immobilizing AChE through glutaraldehyde cross-linking attachment to recognize pesticides selec-tively. Before the detection, the enzyme membrane was quickly fixed on the surfaces of modified electrode with O-ring to prepare an amperometric acetyl-cholinesterase biosensor for organophosphate pesticides. The fabrication procedures were characterized by cyclic voltammetry and amperometric i-t curve. The electrochemical behaviours of three modified sensors were compared, and the results showed that AChE-PB/GCE possessed higher oxidation peak current at a lower potential. Based on the inhibition of organophosphorus pesticides to the enzymatic activity of AChE, using dichlorvos as model compound, the sen-sitivity of three modified biosensors were compared, the results showed that the detection limit of AChE-PB/ GCE was lowest.
Biosensor Acetylcholinesterase Pesticide residue Modified electrode
Xia Sun Xiangyou Wang Wenping Zhao Shuyuan Du Qingqing Li Xiangbo Han
School of Agricultural and Food Engineering, Shandong University of Technology,Zibo 255049, Shandong College of Computer Science and Technology, Shandong University of Technology,Zibo 255049, Shandong
国际会议
南昌
英文
16-24
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）