Chlorimuronethyl Resistance Selectable Marker Unsuited for the Transformation of Rice Blast Fungus(Magnaporthe Grisea)
Chlorimuronethyl resistance gene is increasingly used as a selectable marker for transformation, especially fungal transformation. Magnaporthe grisea is an important model organism for investigating fungal pathogenicity, and Agro-bacterium tumefaciensmediaxed transformation (ATMT) is used for functional mutagenesis of the fungus. However, our results showed that rice blast strains collected from infectious rice fields have highly conserved resistance to chlori-muronethyl, even comparable to transformants which carrying chlorimuronethyl resistance genes as selectable marker in laboratory conditions. PCR results showed that all tested field strains presented the amplified products of the same size as the selectable marker amplified from plasmid carrying chlorimuronethyl gene. Se-quence analysis of PCR products amplified from field strains confirmed that field strains harbored the highly identity homolog of chlorimuronethyl resistance gene. Blast search in GenBank suggested that the fragment is presenting in reference genome sequence of 70-15, but it is not a wide-spread gene in other organisms, excepted for Herpetosiphon aurantiacus. Although the origin and reason of the conserved chlorimuronethyl resistance gene in field isolates of blast fungus is unclear, the ecological function of the gene is noteworthy.
Magnaporthe grisea chlorimuronethyl resistance gene selectable marker fungi transformation
Chang Qing Yang Jing Liu Lin Su Yuan Li Jinbin Zhu Youyong Li Chengyun
Key Laboratory of Agro-biodiversity and Pest Management of the Education Ministry of China,Yunnan Ag Key Laboratory of Agro-biodiversity and Pest Management of the Education Ministry of China,Yunnan Ag Key Laboratory of Agro-biodiversity and Pest Management of the Education Minstry of China,Yunnan Agr
国际会议
南昌
英文
335-342
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）