Nitrogen Status Determination of Rice by Leaf Chlorophyll Fluorescence and Reflectance Properties

摘要：

An experiment was conducted under pot-culture conditions to determine rice nitrogen (N) status by leaf chlorophyll fluorescence and reflectance properties. Three rice cultivars, Zhejing 22, Zhejing 29 and Xiushui 09 were transplanted in 45 twelveliter pots filled with local paddy soil. Each had three nitrogen rate, 0, 75 and 150 kg N ha1. Fertilization application was carried out in tillering period and spectral reflectance, chlorophyll fluorescence and nitrogen content of the uppermost, fully expanded leaves were determined in jointing stage. Spectral reflectance indices R550 (Reflectance at 550 nm), REP (Red edge position), NRI (Nitrogen reflectance index), and PRI (Photochemical reflectance index) were well correlated with leaf nitrogen content of rice with the R2 0.628-0.751**, 0.771-865**, 0.558-0.813** and 0.347*-0.589**. Chlorophyll fluorescence parameters Fv/Fm (Maximal photo-chemical efficiency of PSII) rather than Fo (Initial fluorescence), Fm (Maximal fluorescence) and Yield (Light adapted quantum yield) also can predict leaf nitrogen status, but the determine coeffi-cient was lower in comparison with spectral indices REP, R550 and NRI. Reflectance measurements show better performance in estimating nitrogen status than chlorophyll fluorescence methods. There was close linear relation between Fm and leaf spectral reflectance in the range of 400-1100 nm. In conclusion, leaf reflectance indices R550, REP, NRI, PRI and chlorophyll fluorescence parameters Fv/Fm may be useful for rapid, timely and non-destructive estimation of rice leaf N status.

关键词： Nitrogen Status Leaf Reflectance Chlorophyll Fluorescence Rice REP Fv/Fm

作者: Hao Hu Kefeng Zheng Xiaoming Zhang Yanting Lu Hao Zhang

作者单位: Institute of Digital Agriculture, Zhejiang Academy of Agriculture Sciences, Zhejiang, Hangzhou 31002 Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agriculture Sciences,Zhej

会议类型: 国际会议

会议名称: The 4th IFIP International on Computer and Computing Technologies in Agriculture and the 4th Symposium on Development of Rural Information(第四届国际计算机及计算机技术在农业中的应用研讨会暨第四届中国农业信息化发展论坛 CCTA 2010)

会议地点: 南昌

会议语种:英文

页码: 1207-1211

在线出版日期: 2010-10-22（万方平台首次上网日期，不代表论文的发表时间）