Novel Method for ELISA Traces Pipetting System by Air Displacement Pipetting
In the Enzyme-linked immunosorbent assay (ELISA) test, many steps need traces pipetting. The ELISA test results will be different when we use different pipetting ways. Our traces pipetting system is based on the air displacement pipetting principle, comparable to the functioning of hand pipettes. It is applied pressure sensor to realize pressure-based liquid level detection (pLLD) and aspiration monitoring. The monitored system can distinguish the following situations: (1) a correct aspiration; (2) cup empty; (3) tip-blocked; (4) bubbles. Using the air displacement principle into traces pipetting can avoid contamination or dilution by system liquids, and problems with corroded tubing, pumps, etc. It applied pressure sensor to realize pLLD and aspiration monitoring. The results of the real-time monitor module on air displacement pipetting show that the traces pipetting system can agilely distinguish the different liquid pipetting situations. The method of air displacement pipetting offered an effective way for ELISA traces pipetting system.
ELISA air displacement pipetting traces pipetting pressure liquid level detection
ZHU Lianqing LI Hong GUO Yangkuan
School of Photoelectronic Information & Communication Engineering, Beijing InformationScience & Tech School of Photoelectronic Information & Communication Engineering, Beijing Information Science & Tec
国际会议
湘潭
英文
252-256
2011-07-19（万方平台首次上网日期，不代表论文的发表时间）