Anti-tumor Immunity Induced by Cross-link Complex Alpha-fetoprotein and Glycoprotein 96
Purposes: To study the ability of gp96 to induce specific CTL response and its protective effect against AFP-producing tumor, a recombinant vaccine alpha-fetoprotein (AFP)-glycoprotein (gp96) complex was constructed. Material/Methods: A recombinant peptide vaccine was constructed by conjugating mouse alpha-fetoprotein to glycoprotein 96. By way of intracutaneous injection, mice were primed and boosted with recombinant vaccine mAFP/gp96, whereas single mAFP or gp96 injection as controls. The ELISPOT and ELISA were used to measure the frequency of cells producing the cytokine IFN-gama in splenocytes and the level of anti-AFP antibody in serum from immunized mice respectively. In vivo tumor challenge was carried out to assess the immune effect of the recombinant vaccine. Results: By recombinant mAFP/gp96 vaccine immunization, the number of splenic cells producing IFN-gama and the level of anti-AFP antibody in serum were significantly higher in mAFP/gp96 group than those in mAFP and gp96 groups (122.50±9.30 IFN-gama spots/106 cells vs 46.40±10.32 IFN-gama spots/106 cells, 12.14±7.33 IFN-gama. spots/106 cells, P<0.01; 164.52± 11.22 ug/mL vs 56.32±8.23 ug/mL, 7.56±3.47 ug/mL, p< 0.01). The tumor volume in mAFP/gp96 group was significantly smaller than that in mAFP and gp96 groups (32.46±6.35 mm3 vs 384.16± 11.43 mm3, 832.54± 12.72 mm3, P< 0.01). Conclusions: The study further confirmed the function of glycoprotein 96s immune adjuvant. Sequential immunization with recombinant mAFP/gp96 vaccine could generate effective specific antitumor immunity on AFP-producing tumor. The recombined mAFP/gp96 vaccine may be suitable for serving as an immunotherapy for hepatocellular carcinoma.
glycoprotein96 (gp96) alpha-fetoprotein (AFP) recombinant vaccine immunity
Xiaoping Wang Huanping Lin Qiaoxia Wang
lab of Molecular Pathology Shaanxi University of Chinese Medicine Xianyang 712046, China Department of Infectious Diseases Xian Jiaotong University Affiliated Xian Central Hospital Xian
国际会议
三亚
英文
19-22
2011-03-25（万方平台首次上网日期，不代表论文的发表时间）