A Double Antibody Sandwich ELISA Assay for the Detection of Salmonella in Food Original Samples
Development of monoclonal antibody based sandwich ELISA for rapid detection of Salmonella and optimization of enrichment procedures to increase the sensitivity. Spleen cells from Balb/c mice immunized with flagellin (H=d) antigen of S. typhi were fused with NSO myeloma clones for preparation of mAbs and New Zealand rabbits were used to produce polyclonal antibody. Different enrichment broths were also evaluated. Out of six specific clones to H=d antigen, mAb L2D3G6 was chosen to establish the sELISA curve, which had the detection limit of 104-105 CFU of S. typhi, and the incubation time could be shorten to 4 h. Of the various broths, BPW was found to yield maximum ELISA values. When tested in artificially inoculated food samples, this assay could detect 102 S. typhi CFU/mL within 10 h from various food rinses (meat, vegetable) and milk samples. In comparison to culture, the enrichment-sELISA is rapid and sensitive, and may be used as a rapid screening procedure for environmental monitoring during outbreak situation.
Salmonella S,typhi Monoclonal antibody Sandwich ELISA
Liu Junwei Jiang Jinqing An Zhixing Fan Guoying Liu Liyan Wang Lirong
College of Animal Science. Henan Institute of Science and Technology, Xinxiang, Henan, 453003, China
国际会议
2011 International Conference on Future Environment and Energy(ICFEE 2011)(2011年未来环境与能源大会)
三亚
英文
231-234
2011-03-25（万方平台首次上网日期，不代表论文的发表时间）