Studies on Preparation and Characterization of Lomefloxacin Artificial Antigen
An icELISA immunoassay for determination of Lomefloxacin (LOM) residue has been developed. For this purpose, NHS ester technology was employed to synthesize the immunogen and coating antigen of LOM. Infrared spectra and SDS-PAGE identification showed that the artificial antigen was conjugated successfully. Based on the square matrix titration, an icELISA method was established. The dynamic range in assay buffer was from 0.06 to 98.6 ng/mL, with LOD and ICM value of 0.04 ng/mL and 1.8 ng/mL, respectively. Except for a little cross-reactivity (6.2％) to Ciprofioxacin, this assay showed negligible cross-reactivity to other FQs tested. When spiked in milk, the regression equation was y = 0.9357 x + 0.1333, with a correlation coefficient (R2) of 0.9942. The results suggest that the artificial antigen was synthesized successfully, and the established immunoassay could be used for detecting of Lomefloxacin residues in food samples.
Lomefloxacin Fluoroquinolones Artificial antigen polyclonai antibody Indirect competitive ELISA
An Zhixing Jiang Jinqing Zhao Kun Chang Xinyao Zhang Haitang
College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan,453003,China
国际会议
上海
英文
300-303
2011-03-11（万方平台首次上网日期，不代表论文的发表时间）