Synthesis and Identification of Norfloxacin Artificial Antigen
A multiresidue immunoassay method for determination of Fluoroquinolones(FQs)residues has been developed．For this purpose,NHS ester technology was employed to synthesize the immunogen and coating antigen of Norfloxacin(NFLX)．SDS—PAGE,UV-visible spectra and Infrared spectra identification showed that the artificial antigen was conjugated successfully．Based on the square matrix titration,an icELlSA method was established．The dynamic range in assay buffer was from 0.038 to 112.8 ng/mL, with LOD and IC50 value of 0.02 ng/mL and 1.2 ng/mL， respectively．This assay showed a high crossreactivity to Ciprofloxacin (86％). Enrofloxacin(75％),Difloxacin (63％)， Sarafloxacin(57％)and Pefloxacin(33.8％)．The chemical effects on assay performance showed that the physiological pH (7.4in assay buffer pursued the maximum absorbance (Amax) and the most sensitive IC50 values．The results suggest the artificial antigen was synthesized successfully．and the established immunoassay could be used for simultaneous detecting of Norfloxacin,Ciprofloxacin, Enrofloxacin． Difloxacin,Saranfloxacin and Pefloxacin residues in animaloriginal food samples．
Norfloxacin Fluoroquinolones(FQs)：Artificial antigen polyclonal antibody Indirect competitive ELISA
Liu Junwei Jiang Jinqing Zhang Haitang Fan Guoying An Zhixing
College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan,453003,China
国际会议
海口
英文
393-396
2011-02-22（万方平台首次上网日期，不代表论文的发表时间）