Molecular Cloning and Tissue Expression of the Calpainl(CAPN1) Gene in Goat
Calpain 1 gene is closely related with meat quality in livestock and poultry. Based on (he bovine mRNA sequences (GenBank accession number: AF22119), the cDNA of Calpain 1 was amplified successfully for the first time using T-A clone in Tianfu goat. Results showed that the full-length cDNA of Calpainl was 2151 base pair (bp) encoding a protein with 716 amino acid residues and contained three conserved domains, a catalytic activity region and a catalytic activity centre. Bioinformatic analysis indicated that the secondary structure was mainly alpha helix and random coil contained rich hydrophilic regions, certain phosphorylation loci and protein kinase A/C loci, caprine Calpain 1 was identical to caprine and other speices, especially in mammals. Tissue expression of the gene was all discovered in seven samples. The expression of the 6-month age was the highest in leg muscle. There was a significantly difference between leg muscle, longissimusdorsi and other internal organ tissues.
Culpaine1 cDSA clone tissue expression goat
Boyang Zhao Chengli Zheng Diyan Li Gangyi Xu Yong Gong
Institute of Animal Genetics and Breeding, Sichuan Agricultural University, YaAn, Sichuan, 625014 China
国际会议
海口
英文
65-69
2011-02-22（万方平台首次上网日期，不代表论文的发表时间）