Toxicoproteomics in Chorroid PlexusK-Manganese Induced Neurotoxicity
Toxicoproteomics is a newly evolved discipline in toxicology. This study was designed to investigate if sub-chronic Mn exposure led to changes in protein expression and structural damage in the choroid plexus (CP) ,a brain tissue critical to material transport by the blood-CSF barrier (BCB). Male rats (1.5 months old) received daily ip injection of either MnC12 (6 mg Mn/kg) or saline (as controls) for 30,90 or 90 days (allowed for additional 30-day convalescence). The CP tissues in lateral ventricles were collected at each time point. light microscopy and TEM revealed that Mn exposure resulted in flattening and shrinking of the cell layer,cytoplasmic vacuolation,nuclei and chromosome condensation,mitochondrion destruction,microvilli shortening,and partially disconnection in intra- cellular junctions between two adjacent epithelial cells. The structural alteration was mild after 30day exposure,moderate in 60-day animals and severe in 90-day group even with one month recovery,suggesting a long lasting pathological damage even after Mn exposure was ceased. A 2-DE coupled with nanoLC-Q-TOF MS followed by Gene Ontology (GO) annotation analysis showed that among 448 BCB proteins spots detected,32 differentially expressed proteins were identified,of which 27 were up-regulated and five were downregulated Further verification and analysis are in progress. Taken together,these data suggest that Mn in the CP not only damages the structural integrity,but also alters the expression of proteins critical to BCB function.
Guojun LI Kaihua WEI Haiming JING Fan YANG Chaoying ZHAO Iing MA Tuo ZHANG Xinyue ZHANG Jianzhong LIU
Beijing Center for Disease Prevention and Control/ Beijing Research Center for Preventive Medicine,B Beijing Proteome Research Center,Beijing, China
国际会议
天津
英文
67-69
2011-09-20（万方平台首次上网日期，不代表论文的发表时间）