The Synergistic Effect of Chrysin and TNFa on Apoptosis in Hep G2 Cells
(0) Chrysin(5,7-dihydroxyflavone) exists widely in plants,honey and propolis. The anticancer property of chrysin has beendemonstrated though the molecular mechanism is not clear. In this study, we found that pretreatment of chrysin could synergistically promote the cell death induced by TNFa in human hepatoma(Hep G2) cell lines according to the morphological changes and appearance of sub-G1 peak. Then we found the synergistic effect of chrysin and TNFa is related to the activation of caspase 8 and caspase 3. In further exploring of mechanism, we found nuclear transcription factorkappa B (NF-κB) activity was inhibited by the combination of chrysn and TNFα. As the result,the expressions of c-FLTPL and cIAP2 enhanced by TNFa were suppressed by the combination,which are antiapoptotic proteins regulated by NF-κB. All Data from this study thus reveal a novel function of chrysin as an anti-cancer agent and enhance the possibility of TNFα in clinical application.
Xin LI Xingfen YANG Meifen CHEN Fengyan WANG Junming HUANG Xikun XIONG
Center far disease control and prevention of Guangdong Province,776 Xin Gang West Road,Guangzhou,510300,China
国际会议
天津
英文
81-84
2011-09-20（万方平台首次上网日期，不代表论文的发表时间）