Drug-Induced Changes of Topography in Living B Lymphoma Cells: An Atomic Force Microscopy Study
Due to the particular imaging mode, atomic force microscopy (AFM) provides a new technology for characterizing the topography structures of individual living cells under nearphysiological conditions. However, owing to the lack of adequate immobilization method, using AFM to visualize the topography of mammalian suspension cells is still a big challenge. In this paper, a method for imaging living mammalian suspension cells by AFM was presented. In this method, single B lymphoma suspension cells were mechanically trapped by microfabricated pillars which were coated with positively charged poly-L-lysine. Based on this immobilization, the Rituximab-induced topography changes of individual B lymphoma cells were visualized. The roughness of the cell surfaces local area before and after the stimulation of Rituximab was analysed. The force curves obtained on the cell surface before and after adding Rituximab were also analysed. The experimental results indicate the efficacy of the pillar-based immobilization and will facilitate the research of the physiological activities of living mammalian suspension cells.
Mi Li Lianqing Liu Ning Xi Yuechao Wang Zaili Dong Yoshikazu Hirai Osamu Tabata Xiubin Xiao
State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shen State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shen State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shen Department of Micro Engineering, Kyoto University, Kyoto, Japan Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
国际会议
长春
英文
1-4
2011-08-29（万方平台首次上网日期，不代表论文的发表时间）