Toxic Effect on Tissues and Differentially Expressed Genes in Hepatopancreas Identified by Suppression Subtractive Hybridization of Freshwater Pearl Mussel (Hyriopsis cumingii) Following Microcystin-LR Challenge
Microcystins are a family of potent hepatotoxins produced by freshwater cyanobacteria and can cause animal intoxications and human diseases. In this study, the effect of microcystin-LR (MC-LR) on the tissues of freshwater pearl mussel (Hyriopsis cumingii) was evaluated and differentially expressed genes in the hepatopancreas of the mussel exposed to MC-LR were identified. HPLC analysis of cell extracts from various tissues of the mussel indicated that the hepatopancreas had the highest MC-LR levels (55.78 6 6.73 lg g21 DW) after15-day exposure. The MC-LR concentration in gill or muscle was an order of magnitude less than in hepatopancreas or gonad. Subtractive cDNA library was constructed by suppression subtractive hybridization (SSH), and _400 positive clones were sequenced, from which 98 high quality sequences were obtained by BLAST analysis. The screening identified numerous genes involved in apoptosis, signal transduction, cytoskeletal remodel, innate immunity, material and energy metabolism, translation and transcription which were extensively discussed. The results of this study add large amount of information to the mussel genome data, and for the first time present the basic data on toxicity effect of MC-LR on mussel.
Ziyan Yang Hongjuan Wu Yuan Li
School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei Province 430074, China
国际会议
武汉
英文
28
2011-04-05（万方平台首次上网日期，不代表论文的发表时间）