Isolation from Tannery Wastewater and Characterization of Bacterial Strain Involved in Nonionic Surfactant Degradation
A bacterial strain OPQa3 capable of utilizing nonylphenol polyoxyethylene (NPnEO) as sole carbon source was isolated from water samples collected from tannery waste treatment plant suffered long-time application of NPnEO by enrichment method. It was preliminarily identified as Brevundimonas sp. (most similar to Brevundimonas diminuta (EU434566.1)), according to the observation of morphological characteristics, physiological-biochemical tests and the similarity analysis of its 16S rDNA gene sequence. Inoculated 2% of OPQa3 suspension, to give a final OD600 of approximately 0.70, to inorganic salt medium in which the initial concentration of NPnEO was 746 mgL-1, degradation test showed that, the degradation rate of strain OPQa3 was 84.5% within 120 hours, the optimum temperature was 30℃ while the optimum pH value was about 7, and the growth period was of 24 hours.
YAN Binghua YANG Haijun WEI Jianhong LUO Lin
College of Resources & Environment, Hunan Agricultural University, Changsha, China College of Bio-safety & Technology, Hunan Agricultural University, Changsha, China College of bioscience & biotechnology, Hunan Agricultural University, Changsha, China
国际会议
哈尔滨
英文
22-26
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）