Expression and Functional Analysis of BjMT-2, a Metallothionein Type-2 from Brassica juncea, in E. coli

摘要：

The expression and functional analysis of plant metallothionein type 2 from Brassica juncea were achieved in E. coli. The BjMT-2 cDNA was cloned into the pETM-20 vector fused to the Thioredoxin gene and induced to E. coli. Northern blot and SDS PAGE analysis showed, respectively, that the transcription of the TrxA::BjMT-2 fusion gene and the expression of the fusion protein were predominately in E. coli cells after IPTG induction. The BjMT-2 in the crude extraction prepared from the E. coli cells bearing TrxA::BjMT-2 vector were reacted with the antibody directed against BjMT-2. The E. coli cells exhibited an increased tolerance against Cu2+and Cd2+after BjMT-2 expression.

作者: SONG Yuzhen LI Pingping DU Yejie

作者单位: Northeast Forestry University Forestry College, Harbin 150040,China Alkalisoil environmental science center, Hexing Road 26, Harbin 150040, China Heidelberg university HIP, D-69120-Heidelberg, Germany

会议类型: 国际会议

会议名称: 2011 International Conference on Environmental Biotechnology and Materials Engineering(2011年环境生物技术与材料工程国际会议)

会议地点: 哈尔滨

会议语种:英文

页码: 65-69

在线出版日期: 2011-03-26（万方平台首次上网日期，不代表论文的发表时间）