Development of Pinus koraiensis SSR primers based on EST-SSR information technology

摘要：

A total 408 SSRs were distributed in 18,181 ESTs sequences in Pinaceae in NCBI (National Center for Biotechnology Information) searched by SSRIT software, accounting for 2.24% of the whole EST sequences. We designed 132 pairs of EST-SSR primers by primer3. Of the designed 132 pairs, 29 pairs were able to produce an amplification product in the 10 Pinus koraiensis DNA samples, but only five primers in the 29 pairs exhibited polymorphism. Dinucleotide repeats were the most common repeat class. The repeated primitives of dinucleotide were 10, accounting for 52.73% of the whole repeated primitives;the repeat numbers were 87. The second most common repeat class was trinucleotide. The repeated primitives of trinucleotide were 27, accounting for 42.27% of the whole repeated primitives, and repeat numbers were 78.

作者: Xin Sui Fujuan Feng Dan Zhao Minmin Chen Shijie Han

作者单位: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China Graduate Universit Northeast Forestry University, Harbin 150040,China Northeast Forestry University, Harbin 150040, China Institute of Applied Ecology, Chinese Academy of Sciences,Shenyang 110016, China Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016, China

会议类型: 国际会议

会议名称: 2011 International Conference on Environmental Biotechnology and Materials Engineering(2011年环境生物技术与材料工程国际会议)

会议地点: 哈尔滨

会议语种:英文

页码: 259-266

在线出版日期: 2011-03-26（万方平台首次上网日期，不代表论文的发表时间）