Scavenging of Nitrite and Inhibition of Nitrosamine Formation by Laoying Tea (Litsea Coreana L.) Extracts
50% (V/V) ethanol extract from laoying tea (Litsea coreana Level.) was fractionated by liquid-liquid partition using ethyl acetate, n-butanol and water, respectively. The activities of nitrite scavenging (NS) and nitrosamine formation inhibiting (NFI) of three different fractions have been investigated in vitro systems. In the above assays, all the fractions showed NS and NFI potentials to varying degrees. With regard to NS activity, fractions were effective in the order: ethyl acetate>n-butanol>water fraction. EC50 values for inhibiting ability on nitrosamine formation for ethyl acetate, n-butanol and water fraction were 0.170, 0.554 and 0.725 mg/mL, respectively. Total flavonoids could be the major NS and NFI components and were in the order: ethyl acetate>n-butanol>water fraction. Based on EC50 values, laoying tea especially ethyl acetate-soluble fraction may have a preventive effect against carcinogenesis induced by nitrosamines.
Hongfang Ji Haiyan Zhang Lingwen Zhang Mingduo Yang Yuan Zhang
School of Food Science, Henan Institute of Science and Technology, Xinxiang, P.R.China College of Life Science, Henan University, Kaifeng, P.R.China School of Food Science, Henan Institute of Science and Technology, Xinxiang, P.R.China Institute of Institute of Food Engineering, Harbin University of Commerce, Harbin,P.R.China School of Food Science, Henan Institute of Science and Technology,Xinxiang, P.R.China
国际会议
哈尔滨
英文
452-456
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）