Study on mating system of Pinus koraiensis in natural population based on cpSSR technology
Chloroplast simple sequence repeat (cpSSR) technique was firstly used to study mating system of natural Pinus koraiensis population. Nine pairs of primers with clear spectrum bands, high stability and polymorphism were selected from 70 pairs of cpSSR primers to analyze the mating systems of 28 individuals. 14 polymorphic loci were detected in the nine pairs of primers. The multi-locus (tm) and single-locus (ts) outcrossing rate were 0.966 (SD=0.000) and 0.939 (SD=0.000), respectively, which were slightly higher than those of other tree species. The constant index (F=-0.035) was below zero, indicating the occurrence of excessive heterozygote. Moreover, inbreeding index (tm-ts=0.027;SD=0.000) was close to zero, indicating an insignificant inbreeding. MLDT analysis showed that multi-locus outcrossing rate differed from single-locus outcrossing rate in mating systems of 28 individuals, but the differences were not statistically significant.
Fujuan Feng Dan Zhao Xin Sui Xiaoyan Sun
Northeast Forestry University, Harbin 150040, China Northeast Forestry University,Harbin 150040, China Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China Northeast Forestry University, Harbin 150040,China
国际会议
哈尔滨
英文
700-704
2011-03-26(万方平台首次上网日期,不代表论文的发表时间)