Cloning Na+/H+ Antiporter Gene (nhaA) and Analysis of Function in Soybean
The osmotic stress and ion toxicity caused by high salt is one of the major abiotic stress factors that affect the crop growth and development. Na+/H+antiporter is the key factor in the salt-stress tolerance in organism. It can maintain normal growth and metabolism of organism under high salt stress. PCR was per-formed to clone a plasma membrane Na+/H+antiporter gene nhaA from Pseudomonas stutzeri, and the plant expression vector pB112l-nhaA was constructed. NhaA gene was trans-formed into soybean growing point via Agrobacterium-mediated transformation. PCR, Southern blot analysis showed that nhaA gene had been integrated into the soybean genome. Salt resistance analysis showed that the relative electronic conductivity of the transformed plants plasma membrane was signify-cantly lower than that of the control under salt stress. While the content of chlorophyll and praline in the transformed plants were significantly higher than that in the controls. The expression of nhaA gene increased the salt stress tolerance of the transgenic soybean and provided excellent candidate genes for improving salt tolerance of crops.
Wang Quanwei Chen Liang Zhang Hailing
Life Science and Technology Institute,Harbin Normal University,Harbin,150025,China Heilongjiang Provice Seed Administration, Harbin,150008,China Institute of Pratacultural Sciences,Heilongjiang Academy of Agricultural Sciences,Harbin,150086,Chin
国际会议
哈尔滨
英文
744-747
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）