Isolation and characterization of laccase activity in a novel Bacillus amyloliquefaciens LC02

摘要：

Bacterial strains exhibiting laccase activity were isolated from the forest soil. A strain LC02 with syringaldazine oxidation ability was obtained using enrichment medium supplemented with copper ions. The isolated strain was identified as Bacillus amyloliquefaciens using physiological and biochemical tests as well as 16S rDNA sequence analysis. The characterization of spore laccase activity was investigated. The result showed that the optimum pH and temperature of the enzyme was 6.6 and 70℃, respectively. A great thermostability was observed for the spore laccase at 70℃. Laccase activity was strongly inhibited by 0.1 mmol/L NaN3, dithiothreitol and cvsteine.

作者: Jun-Bo Pan Min Zhao Lei Lu Mei-Hui Du Guo-Fu Li Jun Li Tian-Nv Wang Xiao-Liang Tang

作者单位: College of Life Sciences, Northeast Forestry University, Harbin 150040, P.R.China College of Agricul College of Life Sciences,Northeast Forestry University, Harbin 150040, P.R.China College of Life Sciences, Northeast Forestry University,Harbin 150040, P.R.China College of Life Sciences,Northeast Forestry University,Harbin 150040, P.R.China College of Agriculture,Northeast Agricultural University, Harbin 150040,P.R.China

会议类型: 国际会议

会议名称: 2011 International Conference on Environmental Biotechnology and Materials Engineering(2011年环境生物技术与材料工程国际会议)

会议地点: 哈尔滨

会议语种:英文

页码: 773-777

在线出版日期: 2011-03-26（万方平台首次上网日期，不代表论文的发表时间）