Overexpression of TrxA::BjMT2 (Thioredoxin::Metallothionein type 2 from Brassica Juncea) fusion protein in Escherichia coli and in vitro cleavage by TEV protease

摘要：

BjMT2 cDNA clone gene isolated from Brassica juncea was constructed in pETM-20 and expressed in E.coli as a TrxA::BjMT2 fusion protein. After affinity chromatography and cleavage from the TrxA domain, pure BjMT2 protein was obtained which strongly reacted with the thiol reagent monobromobimane. The amino acid sequence determined by mass spectrograph revealed the polypeptide contains 80 amino acids and is full of 8 and 6 cysteines with CC, CXC and C-XX-C motifs clustered near -N and -C terminus, respectively.

作者: Song yuzhen Li pingping Du yejie

作者单位: Northeast Forestry University Forestry College, Harbin 150040, China Alkalisoil environmental science center, Hexing Road 26, Harbin 150040, China Heidelberg university HIP, D-69120-Heidelberg, Germany

会议类型: 国际会议

会议名称: 2011 International Conference on Environmental Biotechnology and Materials Engineering(2011年环境生物技术与材料工程国际会议)

会议地点: 哈尔滨

会议语种:英文

页码: 947-951

在线出版日期: 2011-03-26（万方平台首次上网日期，不代表论文的发表时间）