Fermentative production of Harpin protein by a recombinant E. coli strain and its toxicity determination

摘要：

Harpin protein was produced by a recombinant E. coli BL21(DE3)/pET30a(+)hrpNEcc in a 1-ton fermentor, and acute oral toxicity test in rats and guinea pig skin sensitization test for Harpin protein were carried out in order to determine its toxicity. The experimental results show that the protein production is up to 4.78 g/L after 16-18 h of fermentation, and the production cost of this protein is 1.75 yuan/g. Harpin protein produced by the recombinant E. coli strain is non-toxic (LD5o> 5000mg/kg) and has a low allergenicity (rate of sensitization is 0％). The results obtained in this study lay the foundation of the industrial manufacturing and the application on agriculture for Harpin protein.

作者: Min Wang Xiaohong Sun Tianlei Qiu Meilin Han Xuming Wang

作者单位: Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

会议类型: 国际会议

会议名称: 2011 International Conference on Environmental Biotechnology and Materials Engineering(2011年环境生物技术与材料工程国际会议)

会议地点: 哈尔滨

会议语种:英文

页码: 971-974

在线出版日期: 2011-03-26（万方平台首次上网日期，不代表论文的发表时间）