Optimization of Fermentation Conditions for Cold-active Cellulase Production by Response Surface Methodology
The design of an optimum and cost-efficient fermentation conditions for production of cold-active cellulase by Penicillium cordubense D28 was attempted by using response surface methodology (RSM). Based on the Plackett-Burman design, temperature, seed age and inoculum size were selected as the most critical condition. Subsequently, they were investigated by the Box-Behnken design. Results showed that the maximum cold-active cellulase activity of 110.4U/mL was predicted when the coded values of temperature, seed age and inoculum size were -0.495, 0.455, and 0.253, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective temperature, seed age and inoculum size of 17℃, 29h and 12.5％. The maximum cold-active cellulase activity of 219.9 U/mL was obtained based on the optimized fermentation conditions with further verified the practicability of this optimum strategy.
Shuo Dong Naiyu Chi Qingfang Zhang
Bioengineer College, Da Lian University, Liaoning Technology of Marine Microbiological Engineering Research Center, Da Lian, Liao Ning 11662 , China
国际会议
哈尔滨
英文
1025-1029
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）