Application of SRAP and ISSR molecular markers in genetic diversity of DaXinganling area wild Auricularia auricula
A genetic diversity analysis was conducted, of 14 wild Auricularia auricula strains and 6 cultivated strains from DaXinganling region by SRAP and ITS markers. Using PCR-SRAP system, we selected 9 pairs of primers for the wild and cultivated strains respectively, and analyzed their genetic diversity through clustering analysis. The results were as follows: 88 fragments were amplified, and the polymorphic bands were 76. The PIC (polymorphism information content) value of these markers varied from 0.048 to 0.918, averaging 0.599. Kinship of Auricularia auricula strains was determined by analyzing their ITS sequences. The software ClustalX 1.83 and MEGA 4.1 were used to conduct the phylogeny analysis. The results were as follows: ITS 1, 5.8 S, ITS2 had as many genetic loci as 52.1％. Cluster analysis of the two kinds of markers were congruous and there were more genetic diversities in wild strains than in cultivated strains. SRAP and ITS techniques can be used to analyze the genetic diversity in the future study.
Hua -Jing Liu Xiu-hong Xu Xiao Yu Ting-Bo Jiang Xue-Zhong Zou
College of Forestry, Northeast Forestry University, Harbin, China, 150040 College of Resources and E College of Resources and Environmental Sciences, Northeast Agricultural University, Harbin, China, 1 College of Forestry, Northeast Forestry University, Harbin, China, 150040
国际会议
哈尔滨
英文
1118-1122
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）