Study on the technical conditions of purifying v-linolenic acid from evening primrose oil by urea inclusion
The urea inclusion was carried out to purify γ-linolenic acid (GLA). The single-factor, double-factor and orthogonal test were employed to optimize technical conditions. Single-factor tests showed that the best ratio of FFA: urea: alcohol was 1:3:8, the inclusion temperature was -15or-20℃ and the duration time was 24 hours. Double-factor test indicated the optimal combination was A2B2 or A2B1.The best combination of the orthogonal test was A1B2C2D2 or A4B2C2D2. The ratio of filtrate and urea was 6:2 or 8:3, at the method of isolation was NO.Ⅱ. Using urea inclusion, GLA purity was raised over 90％ with a yield of 80％.
Gang Xue Ying Wang Fengxia Liu
Department of Biology and Chemical Engineering, Nanyang Institute of Technology, Nanyang, Henan, P.R College of life science and technology, Huazhong Agriculture University, Wuhan, Hubei P.R.China
国际会议
哈尔滨
英文
1148-1152
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）