Study on the Optimal Isolation Technique of Superoxide Dismutase from Pig and Bovine Blood Block
Double, three factor-test and orthogonal designs were employed to study the effect of protective agent, membranolytic agent, activating agent, denaturalization temperature and denaturalization time on SOD extracted from pig and bovine blood block. The results of double and three factor-tests show that the variance between pig and bovine blood block was obviously significant (P <0.01). The mixture protective agent was better than single, the best was 1.5％ galactosylglucose+0.5％ β-cyclodextrin+0.25％ PEG, and the better membranolytic agent was 3％ Triton X-100+0.5％ n-butyl alcohol for bovine blood, and 3％ Triton X-100 for pig blood. The optimal dosage of protective agent was 20％ of blood block weight, the optimal dosage of membranolytic agent was 15-20％ of blood block weigh, and the best optimal dosage of activator was 2.5％. The best optimal combination of orthogonal experiment for bovine blood block was A2B2C2, and the best optimal combination of pig blood was A1B1C2. Under those conditions, the activity of SOD exceeded 3000 U/mg protein.
Fengxia Liu Jiancheng Luo Ying Wang Gang Xue
Department of Biology and Chemical Engineering, Nanyang Institute of Technology, Nanyang, Henan, P.R College of life science and technology, Huazhong Agriculture University, Wuhan, Hubei P.R.China
国际会议
哈尔滨
英文
1224-1228
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）